Nirma University Journal of
Pharmaceutical Sciences
An International Peer Reviewed Journal
Pharmaceutical Sciences
An International Peer Reviewed Journal
Author : Ravisinh Solanki, Nrupesh Patel, Misari Patel, Charmy Kothari Department of Pharmaceutical Analysis, Institute of Pharmacy, Nirma University, Ahmedabad, Gujarat
A simple, sensitive, rapid, accurate and precise absorption ratio method (Q value analysis method) has been developed for simultaneous estimation of loteprednol etabonate and levofloxacin in combined dosage form. Ratio of absorbance at two selected wavelengths was calculated. First wavelength is absorption maxima of respective drug and second wavelength is iso-absorptive point at which both drugs give same absorbance. Loteprednol etabonate showed absorbance maxima at 269.29 and levofloxacin showed absorbance maxima at 298.5 nm in methanol. The iso-absorptive point of loteprednol etabonate and levofloxacin was found to be at 269.29 nm. Linearity was constructed in the concentration range of 5-25 μg/mL. Promising values of correlation coefficient for LE (R<sup>2</sup>=0.998) and levofloxacin (R<sup>2</sup>=0.999) proves that method is linear. Furthermore, the method was successfully validated in terms of various validation parameters as per ICH Q2 (R1) guidelines. The developed method was successfully applied for estimation of both loteprednol etabonate and levofloxacin from its eye drops. Mean % recovery indicated that no interference was observed from excipients present in the formulation. Therefore, developed method can be routinely applied for simultaneous estimation of loteprednol etabonate and levofloxacin from its pharmaceutical dosage form.
